激发光源用于大豆花叶病毒研究

南京师范大学近日在《Agronomy 》发表文献《Genome-Wide Analysis of Soybean Mosaic Virus Reveals Diverse Mechanisms in Parasite-Derived Resistance》，文献中实验使用了上海路阳仪器有限公司生产销售的LUYOR-3415RG便携式双波长荧光蛋白激发光源，用于观察绿色荧光蛋白GFP和红色荧光蛋白RFP在叶片上的表达。LUYOR-3415RG内置了440-460nm蓝光波段，佩戴LUV-30A荧光观察眼镜，可以观察绿色荧光蛋白在动植物上的表达，同时还内置了520-530nm绿光波段，配合LUV-50A荧光观察眼镜，可以观察红色荧光蛋白（例如：tdtomato，mcherry，dsred2）在动植物上的表达，LUV-495A和LUV-590A拍照滤镜可以配合单反相机进行荧光照片拍摄。

文献摘要：

Plant viruses cause severe losses in agricultural production. Parasite-derived resistance (PDR) offers a promising avenue for developing disease-resistant varieties independent of resistance genes. However, for potyviruses with great agricultural importance, such as soybean mosaic virus (SMV), systematic research on viral genes that can be used for PDR has not been conducted. In this study, we transiently expressed the untranslated region (UTR) or each protein-coding cistron of SMV in Nicotiana benthamiana to evaluate their potential role in conferring PDR. A viral suppressor of RNA silencing (VSR) was also applied to investigate the possible mechanisms of the PDR. The results showed that the transient overexpression of UTR and each cistron of SMV could inhibit SMV infection. The expression of VSR in N. benthamiana leaves could compromise UTR and most of the SMV cistron-mediated inhibition of SMV infection, indicating the involvement of RNA silencing in PDR. In comparison, the expression of VSR could not compromise the PDR conferred by coat protein (CP), P3N-PIPO, cylindrical inclusion (CI), and NIa-Pro, suggesting that these viral cistrons may play roles in PDR at the protein level. These results reveal diverse mechanisms in PDR conferred by different viral cistrons and provide potential gene candidates that can be used for transgenic approaches against SMV.

2.4. Photograph and Microscopy

For assessment of the viral infection in the leaves, the GFP and RFP signals were excited using a handheld LED lamp LUYOR-3415RG (Shanghai Luyor Instrument Co., Ltd., Shanghai, China). Subsequently, the fluorescence emitted by GFP and RFP was captured using an LP510 filter or a BP590 filter (LUV-590A, Shanghai Luyor Instrument Co., Ltd., Shanghai, China), respectively, with a digital camera. In order to overlay images of the leaves with different fluorescence, Photoshop CC (version 14.0) was used to extract the RGB signals of fluorescent proteins.

文献地址：https://doi.org/10.3390/agronomy14071457 

LUYOR-3415RG产品介绍：

中文：LUYOR-3415RG双波长荧光蛋白激发光源

英文：Dual Fluorescent Protein Flashlight LUYOR-3415 

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