近期中国热带农业科学院在《Horticultural Plant Journal》期刊上发表了题为《Agrobacterium tumefaciens-mediated transformation of embryogenic callus and CRISPR/Cas9-mediated genome editing in ‘Feizixiao’ litchi》文献,文献中采用了LUYOR-3415RG便携式双波长激发光源用于观察GFP绿色荧光蛋白在荔枝愈伤上的表达。
LUYOR-3415RG便携式双波长激发光源照射荔枝愈伤,有绿色荧光蛋白的发出绿色荧光
文献摘要:
The embryogenic callus was transformed under optimum transformation conditions. After the second round of Hyg screening, resistant yellow callus in 31.58% of the total transformed callus was selected and half was used to induce somatic embryos. Finally, histochemical staining of callus and somatic embryos for GUS gene expression determination and a hand-held lamp (LUYOR 3415RG, Shanghai, China) were used to investigate the expression and resulting fluorescence signals of GFP in resistant transgenic callus and somatic embryos.
文献地址:https://doi.org/10.1016/j.hpj.2023.01.011
LUYOR-3415RG手持式荧光蛋白激发光源可检测转GFP、eGFP、tdtomato、cherry、dsred、dapi、hoechst、cfp、bfp、yfp、venus、lucife等多种荧光蛋白的表达。激发光源激发出绿色荧光时,用黄色滤光镜观测,可检测含绿色荧光蛋白(GFP)的生物;激发光源激发红色荧光时,用红色滤光镜观测,可检测含红色荧光蛋白(DsRed)的生物。LUYOR-3415RG双荧光蛋白激发光源用于检测、筛选转绿色荧光蛋白(GFP)和红色荧光蛋白(DsRed)基因的植物、动物及微生物,如水稻、玉米、斑马鱼、小鼠、细菌、真菌等等, LUYOR-3415RG和LUYOR-3430、LUYOR-3260除了可用于筛选转基因生物外,还可以用于样本的预筛选、辅助解剖、用于珊瑚研究等,
如果您对LUYOR-3415RG感兴趣,我们提供样机免费试用。